Intermediate-size Expanded Access to ONC201 for Patients With H3 K27M-mutant and/or Midline Gliomas

Inclusion Criteria:

1. Patient meets one or more of the criteria below:

Arm A - Closed to further enrollment.

Arm B - Diffuse intrinsic pontine glioma (DIPG) defined as tumors with a pontine
epicenter and diffuse involvement of the pons (at the discretion of the Medical
Monitor, the site may be requested to provide the most recent imaging report(s) to
confirm diagnosis). H3 K27M status does not have to be known or positive for this
arm.

Arm C -

1. Patients with primary spinal glioma that is positive for the H3 K27M mutation
(performed in a laboratory with Clinical Laboratory Improvement Amendments
[CLIA] or equivalent certification). Primary spinal glioma must be documented
in radiology reporting.

OR

2. Patients with diffuse glioma that is positive for the H3 K27M mutation
(performed in a laboratory with CLIA or equivalent certification) AND
radiographic evidence of leptomeningeal disease. Leptomeningeal disease must be
documented in radiology reporting.

Arm D - Closed to further enrollment.

Arm E - Patients with H3 K27M-mutant glioma or a midline glioma of unknown H3 K27M
mutational status who received ONC201 and/or ONC206 from an alternative
(non-Chimerix) source prior to 31 December 2023, as evidenced by supporting
documentation (e.g., medical records, pharmacy receipts, etc.). Other supporting
documentation may be confirmed by the medical monitor. Detection of H3 K27M mutation
should be performed in a CLIA-certified or equivalent laboratory.

Arm F - Patients with H3 K27M-mutant diffuse glioma who have progressed during or
after completion of frontline radiotherapy. Detection of H3 K27M mutation should be
performed in a CLIA-certified or equivalent laboratory. Enrollment in this arm will
be individually evaluated by the Sponsor and requires at least 3 days for review.

2. Disease status:

Arm B - Patient is not required to have radiographic or clinical evidence of
progressive disease.

Arm C - Patient must have progressive disease as defined by Response Assessment in
Neuro-Oncology (RANO) criteria or have documented recurrent glioma on diagnostic
biopsy.

Arm E - Not applicable.

Arm F - Patient must have progressive disease as defined by RANO criteria or have
documented recurrent glioma on diagnostic biopsy.

3. Prior radiotherapy:

Arm B - Patient must be at least 14 days from completion of radiotherapy.

Arm C - Patient must be at least 90 days from completion of frontline radiotherapy
and at least 14 days from reirradiation if applicable.

Arm E - Not applicable.

Arm F - Patient must be at least 90 days from completion of frontline radiotherapy
and at least 14 days from reirradiation if applicable.

4. (Not applicable; criterion removed in Version 5).

5. Patients must weigh at least 10 kg.

6. Washouts:

Arms B, C, and F - From the projected start of scheduled study treatment, the
following time periods must have elapsed from prior anti-cancer treatments: 5
half-lives from any investigational agent, 4 weeks from cytotoxic therapy (except 23
days for temozolomide and 6 weeks from nitrosoureas), 6 weeks from anti-cancer
antibodies (no washout required for bevacizumab), 4 weeks (or 5 half-lives,
whichever is shorter) from other anti-tumor therapies including CAR-T and other
cellular therapies/immunotherapies, and 1 week from devices used to treat cancer.

Arm E - No washouts are required for ONC201 and ONC206. All other anti-cancer agents
need to be discontinued prior to enrollment with the exception of bevacizumab.

7. Magnetic resonance imaging (MRI) of patient's glioma obtained within 28 days prior
to start of ONC201 for Arms B, C, and F.

Arm E: MRI obtained within 8 weeks prior to enrollment.

8. Adequate organ and marrow function as defined below:

1. Absolute neutrophil count ≥1,000/mm3 without growth factor use ≤7 days prior to
treatment (Cycle 1 Day 1 [C1D1])

2. Hemoglobin ≥8.0 mg/dL without red blood cell transfusion ≤3 days prior to C1D1

3. Total serum bilirubin ≤1.5 X upper limit of normal (ULN) (participants with
Gilbert's syndrome may be included with total bilirubin > 1.5 × ULN if direct
bilirubin is ≤ 1.5 × ULN)

4. AST (SGOT)/ALT (SGPT) ≤2.5 X ULN; ≤5 X ULN if there is liver involvement
secondary to tumor

5. Serum creatinine ≤1.5 X ULN (OR creatinine clearance ≥60 mL/min/1.73 m2) Arm E:
Patients with organ and marrow function laboratory values outside the defined
criteria must be approved by the medical monitor.

9. Female patients of childbearing potential must agree to use an effective
contraception method while taking ONC201 and for at least 90 days after the last
dose of ONC201 and must have a negative pregnancy test prior to starting ONC201.
Male patients must be surgically sterile (i.e., >3 months post-vasectomy) or must
agree to use an effective contraception method while taking ONC201 and for at least
90 days after the last dose of ONC201. Determination of effective contraception
methods will be based on the judgment of the Investigator.

10. Ability to understand a written informed consent document, and the willingness to
sign it. At the discretion of the Investigator, a Legally Authorized Representative
(LAR) may consent on behalf of a patient who is unable to provide informed consent
themselves. Assent will be obtained, as appropriate, based on the patient's age.

Exclusion Criteria:

1. Qualifies for participation in an ongoing ONC201 or ONC206 clinical trial.

2. Arms B, C, and F: Previous or current enrollment in an ONC201 or ONC206 clinical
study (including open-label and blinded studies) or expanded access protocol or
previous exposure to ONC201 from any source for the treatment of CNS tumor.

Arm E: Previous or current enrollment in an ONC201 clinical study (including open
label and blinded studies) or expanded access protocol for the treatment of CNS
tumor.

3. Current or planned participation in a study of an investigational agent (including
ONC206) or using an investigational device.

4. (Not applicable; criterion removed in Version 4).

5. Any known systemic infection that, in the opinion of the Investigator, could
compromise the safety of the patient while taking ONC201.

6. Prolongation of QT/QTcF interval (QTc interval >480 milliseconds) using Fridericia's
QT correction formula on two electrocardiograms (ECGs) separated by at least 2 days.

7. A history of Torsades de pointes or heart failure, hypokalemia, or family history of
prolonged QT Syndrome.

8. Concomitant use of medication(s) known to prolong the QT/QTc interval.