Primary endpoints - Incidence of symptomatic and asymptomatic infection with SARS-CoV-2 and other respiratory viruses as determined by molecular (e.g. PCR) and serological testing - Associations between the magnitude and quality of mucosal and serum antibody responses to SARSCoV- 2 and protection from infection with SARS-CoV-2 - Associations between the magnitude and quality of T cell responses to SARS-CoV-2 and protection from infection with SARS-CoV-2 Secondary endpoints - Secondary attack rate and household cumulative infection rate with SARS-CoV-2, influenza, RSV, and other respiratory viruses - Seroincidence and seroprevalence of SARS-CoV-2 a determined by binding antibodies to SARS-CoV-2 spike and nucleocapsid - Presence of risk factors for symptomatic and asymptomatic infection with respiratory viruses - Antibody and T cell kinetics of SARS-CoV-2 following infection - Associations between the magnitude and quality of antibody and T cell responses to seasonal coronaviruses and protection from infection with SARS-CoV-2 - Associations between infection with non-SARS-CoV-2 respiratory viruses and protection from infection with SARS-CoV-2 - Associations between upregulation of gene expression in the mucosa, including interferon stimulated genes (ISGs), and protection from infection with SARS-CoV-2
A prospective, observational cohort study within households in West Coast Region and
Kanifing Municipality, The Gambia. A total of 50 - 70 households will be recruited. All
members of the household will be included other than those who do not meet the
eligibility criteria or decline to consent for the study. Participants will be enrolled
for a total of 52 weeks, undergoing weekly field visits, along with an enrolment visit
(V1), 6-month visit (V2) and 12-month visit (V3), which will all be clinic based. Clinic
visits will collect anthropometric, socio-demographic and clinical data (including risk
for acquisition of SARS-CoV- 2). In addition, a combined throat and nose swab (TNS),
nasal lining fluid (NLF) using a synthetic absorptive matrix (SAM) strip, and a blood
sample will be taken. Weekly visits will be done at the participants' homes or work
place. During these visits, TNS will be collected, along with the presence of symptoms
consistent with respiratory viral infections. In addition, participants who have symptoms
consistent with influenza-like-illnesses (ILI) will have unscheduled visits where
clinical data and a TNS is collected, which will be tested in real time for SARS-CoV-2
using PCR. Participants will be informed of their results within 24 hours after this TNS
sample is collected at unscheduled ILI visits. Positive results will be communicated to
the Ministry of Health for their records and contact tracing. Weekly TNS from all
household members of any positive SARS-CoV-2 case will also be tested in real time for
SARS-CoV-2 for 4 weeks following the most recent SARS-CoV- 2 positive case in the
household. All participants with symptomatic SARS-CoV-2 detected at an unscheduled ILI
visit will be seen at an additional clinic visit (post-covid follow up), where further
TNS, NLF and blood samples will be taken.
Inclusion Criteria:
- Households must:
- be located in Kanifing municipality or West coast region.
- have at least 5 consented members.
- include the household head as a consented participant.
- have household members that includes at least one adult and at least one child.
Participants must:
- provide informed consent/assent according to their age.
Exclusion Criteria:
-
Field study in the West Coast Region and Kanifing Municipality Clinical Services Department, MRCG MRC Unit The Gambia at LSHTM (Fajara) Laboratories at MRC Unit The Gambia at LSHTM (Fajara) Laboratories at The University of Sheffield, UK
Banjul, Western Region, Gambia
Not Provided