The aim of the study is to find the proportion of people in the population of the OlomoucRegion with the presence of IgG (or IgM) antibodies against the S and N antigens of theSARS-CoV-2 virus, therefore people who have already encountered the SARS-CoV-2 virus withmanifest antibody formation, or people who have undergone vaccination against COVID-19.
Study has three study visits. First two visits are three months apart. Second and third
visits are five months apart. On each study visit participant will provide venous blood
sample and saliva sample obtained by gargling with the use of GARGTEST sampling kit.
Participant will also fill in a questionnaire about SARS-CoV-2 infections and
vaccinations. Site staff will measure the body temperature of the patient to exclude a
patient with an acute infection. Blood sample will be used for the assessment of
antibodies and cellular immunity of SARS-CoV-2. Part of the blood will be biobanked for
the future SARS-CoV-2 biomarker determination. Gargling sample will be used for the
demonstration of the pathogen on mucous membranes. Gargle self-sampling using GARGTEST
sampling kit will be done by participants at 14-day intervals between the study visits.
For gargle sample RT-PCR (real time polymerase chain reaction) will be done to determine
the patogen presence.
Procedure: Blood sampling
Venous blood sampling three times on three visits of the study.
Procedure: Gargle sampling
Gargle sampling using GARGTEST sampling kit on three visits of the study and also gargle
self-sampling using GARGTEST sampling kit at 14-day intervals between the study visits.
Other: Questionnaire completion
Patient will complete questionnaire on each visit on SARS-CoV-2 infections and
vaccinations
Diagnostic Test: Body temperature measurement
Body temperature will be measured by healthcare professional at each visit to exclude
patients with acute infection.
Inclusion Criteria:
- Participation in the SARS-CoV-2-CZ-PREVAL study and venous blood examined as part of
this study
- Signed study informed consent and consent to the processing of personal data
- Adults (age 18 and over)
- Willingness to complete the questionnaire
Exclusion Criteria:
• Participant with acute health problems
University Hospital Olomouc
Olomouc, Czechia
Marian Hajduch, MD, PhD, Study Director
Palacky University Olomouc