Introduction: A pandemic such as the SRAS-CoV-2 (COVID-19) has a great negativesocioeconomic impact with very limited therapeutic options. As with any disease, adetailed understanding of its pathophysiological mechanisms is critical for thedevelopment of new therapies. In SRAS-CoV-2, few studies have verified a possiblerelationship of these vasoactive peptide polymorphisms with patient prognosis. Objective:To analyze and relate polymorphisms found in components of vasoactive peptide systems inDNA samples collected from patients diagnosed with SARS-CoV-2 (COVID-19) who developedsevere conditions and patients infected with mild or asymptomatic conditions.Methodology: Cross-sectional, analytical and qualitative study that will be conductedwith approximately 151 participants previously diagnosed with SARS-CoV-2 with mild orasymptomatic forms of the pathology, diagnosed in primary care in the city ofGuarulhos/SP- specifically in the Basic Health Unit of Nova Saúde Bonsucesso- well withparticipants who were diagnosed with the severe forms that required hospitalization in2021. For the collection of biological material, a sterile swab will be used in order tocollect cells from the oral cavity, specifically from the oral mucosa. Expected results:We hope to identify and relate the polymorphisms of vasoactive peptide genes frompatients with mild, asymptomatic or severe forms of SARS-CoV-2 infection, thuscontributing to the understanding of the different clinical evolutions of the disease.
Cross-sectional, analytical and qualitative study that will be carried out with
approximately 151 participants who were previously diagnosed with SARS-CoV-2 with mild
and severe symptoms of the pathology, diagnosed in primary care in the city of
Guarulhos/SP - specifically in the Basic Health Unit Nova Bonsucesso or who have a
history of previous admissions - requiring hospitalization in the year 2021 (which will
be removed from the COVID case monitoring spreadsheet available at the study UBS). Cells
will be collected from the participants' oral cavity using a sterile cotton swab. The
collections will be carried out at the Basic Health Unit Nova Bonsucesso, located in the
city of Guarulhos/SP (mild/asymptomatic cases), under the supervision and responsibility
of the dentist and collaborator of this Leonardo Paroche de Matos project. The Free and
Informed Consent Form will be filled out and signed after guidance on the research and
its subsequent acceptance by the participant.
Other: Analysis of genetic polymorphisms of vasoactive peptides in COVID-19
Evaluate the frequency of the insertion/deletion (I/D) polymorphism in the ACE gene; to
assess the frequency of polymorphism in the bradykinin B2 receptor gene; to assess the
frequency of the polymorphism in the CK-M gene and compare the frequencies of these
polymorphisms between patients with severe and mild conditions.
Inclusion Criteria:
- Individuals over 18 years old
- With or without associated comorbidities: any pathology of the systems:
cardiovascular, respiratory, endocrine, digestive, neurological, psychiatric,
genitourinary
- Diagnosed with COVID-19 through RT-PCR (naso-oropharyngeal swab)
- Who agreed to participate in the research voluntarily
Exclusion Criteria:
- Participants who refuse to participate in the survey
- Who have lesions in the oral mucosa that make the collection of material unfeasible
- Individuals with severe xerostomia
- In cancer treatment.
University Ninth of July
São Paulo, Sao Paulo, Brazil
Guarulhos City Hall
Guarulhos, São Paulo, Brazil
Leonardo Paroche de Matos, Msc
+551124532163
leonardoparoche@gmail.com
José Antônio Silva Júnior, PhD, Study Director
University Ninth of July