The study was carried out on March 15.2021 and the data collection was completed within 6
months. For the randomization of the participants, they were first stratified according
to age and then the participants were selected according to the simple randomization
table and included in the study.The sample of the study was calculated to be at least 48
people for each group, with the power of the test being approximately 80,151%. Ethics
committee approval of the study was obtained from the clinical research ethics committee
of Katip Çelebi University with no: 27 on 11.03.2021. A total of 144 people who met the
inclusion criteria, 48 individuals for each group, were included in the study. The study
was conducted in accordance with the principles of the Declaration of Helsinki.
Participants were included in the study after signing the informed consent form. The
exclusion criteria included having dermatosis (acne, acne rosacea, seborrheic dermatitis)
and/or other dermatological diseases associated with the presence of Demodex mites,
receiving or having been receiving treatment for Demodex-associated dermatosis (topical
and/or systemic), using face wash gel and/or cologne, which prevents the life of Demodex
mites, cosmetic cream with pore-clogging feature, applying disinfectant; being obese
(BMI> 30), consuming regular alcohol use, and taking oral therapy that suppresses the
immune system, or using creams with immunological effects when applied topically on the
face as well as developing any skin complaints with wearing masks, Individuals who wore
N95/FFP2 masks during the whole working hours, and only took off it for less than 1 hour;
(ıı) those who wore a 3-ply surgical mask during all working hours, only took it off for
less than 1 hour and (ııı) people who wore a 3-ply surgical mask for less than 3 days a
week, less than 2 hours a day and spending time mostly at home during the day or plus
those who worked alone all day in their rooms and who wear a 3-ply surgical mask for less
than 1 hour during the day as required. The third group represented the control group
(the control group was comprised of individuals who presented to the hospital during the
study period, since there was no one without mask during the day). Participants were
included in the study after signing the informed consent form. The sociodemographic
characteristics, including age, gender and occupation; cigarette use, alcohol use, the
past medical history, face washing habits (only water, water and soap, non-specific face
wash product) and dermatological examination, whether the skin is oily or dry-normal, and
Fitzpatrick skin typing were determined. In order to detect the presence of Demodex
mites, standard superficial skin biopsy (SSSB) was applied to the face that was wiped
with dry sterile gauze after dropping cyanoacrylate by marking 1 cm2 area on the slide
that was wiped with dry sterile gauze from 3 areas of the face, right cheek, nose and the
mid-forehead. After the slide was left for 60 seconds and slowly removed from the face,
immersion oil was dripped onto the slide and covered with a coverslip, and Demodex mites
were counted first under the light microscope at x10 and then x20 magnification (Demodex
foliculorum and brevis were not differentiated). The process was repeated for each
region. The presence of Demodex mites in each region and its number, if any, were written
on the inquiry form.